Liquid Biopsies & Cell-Free DNA Extractions
Accelerating Diagnostic Discovery, Development, and Delivery
Azenta has developed a fully automated proprietary liquid biopsy workflow involving cell-free DNA (cfDNA) extraction from plasma designed to promote sample quality. The workflow accommodates either whole blood or isolated plasma as a starting material, and can be performed on samples newly shipped to us or samples already stored at an Azenta site. If you are shipping new samples for this service, we recommend using Streck® or PAXgene™ Blood DNA tubes or equivalent for best results. Liquid biopsy and cell-free DNA extraction solutions from Azenta help you accelerate diagnostic discovery, development, and delivery.
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Key Features
Quality
Projects can be processed at labs working compliant to ISO 9001:2015, CAP and/or CLIA certification standards, and unified SOPs across sites.
Speed
Sample processing begins within 24 hours of receiving your sample.
Flexibility
Customizable workflows are adjusted to accommodate clinical research needs, including aliquoting, pooling, and plasma storage.
Preparation for Shipped Samples
Starting Materials Accepted
- Whole blood
- Isolated plasma
Recommended Tubes
- Streck® Blood DNA tubes
- PAXgene™ Blood DNA tubes
- Equivalent tubes to the options above
Sending Options
Following project approval, samples should be mailed by direct courier, such as FedEx or Marken, to one of the lab services facilities embedded within our primary US and EU biorepository locations.
Customization Options
Aliquoting
Customizable cfDNA resuspension buffer and aliquoting strategies
Pooling
Multiple cfDNA extractions are pooled to create combined samples
Plasma Storage
Aliquots of spun plasma can be stored for future extraction
Interested in adding a customized option to your workflow?
Simply mention this when talking with a representative or in your message when placing a request for this service
Cell-Free DNA Extraction Workflow
STEP 1
Receive Sample & Register to LIMS
Sample is either shipped to us or retrieved from our storage. Upon receipt, sample is registered or updated in the Limfinity® Sample Management System software, which supports 21 CFR, part 11 compliance and continually tracks parent/stock sample and child/aliquot relationships.
STEP 2
Sample Processing
Beginning within 24 hours of receiving or retrieving the sample, sample processing follows unified SOPs and ensures consistent sample integrity and quality.
STEP 3
cfDNA Extraction
Cell-free DNA is isolated following our quality-controlled processes, including silica column or magnetic bead-based technologies.
STEP 4
Quality Assessment
Our proficiency-tested methodology includes sample quality checks for DNA recovery rates, concentration, and yield.
STEP 5
Cryopreservation & Storage or Downstream Analysis
Store the sample at Azenta for future use or consider downstream analysis which includes Clinical Sequencing, CLIA Whole Genome Sequencing, Targeted Sequencing, or qPCR with our GENEWIZ Multiomics & Synthesis Solutions team.
Downstream Analysis
with our GENEWIZ Multiomics & Synthesis Teams
Clinical Sequencing
CLIA Whole Genome Sequencing
Targeted Sequencing
qPCR
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