FrameStar® 96 Well Skirted Optical Bottom PCR Plate
96 well skirted plates unique on the market, suitable for use in both microscopy and PCR. Low profile wells, especially recommended for reaction volumes <20µl. Rigid frame eliminates warping and distortion during PCR. Ideal for use with robotic systems.
- PP wells, PC frame; cut corner H1
- <100µl working volume, 180µl total well capacity
- Available barcoded on request
- Ideal for single cell sorting followed by (q)PCR
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Product Details
We have now extended our FrameStar range to include PCR plates with optically clear flat bottom wells for microscopy applications. The FrameStar 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our Individual Access range, 4ti-0970/RA) are currently unique in the market, being suitable for use in both microscopy and PCR.
Optical bottom plates are ideal for applications requiring single cell sorting followed by molecular biology techniques such as (q)PCR and sequencing. The flat bottoms enable excellent stackability, making these plates well suited for small sample volume storage such as compound libraries, with no risk of damaging the seal of the plate below. Additionally, the small well volume enables excellent sample recovery.
Key Features
Quality
- Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
- Stackable
Wells
- Ultra-smooth, uniform, thin-walled polypropylene wells for optimum PCR and real-time (RT-qPCR) results
- <0.1ml (100µl) working volume, 0.18ml (180µl) total well capacity
- Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
- Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction
Frame
- Rigid polycarbonate frame for added mechanical stability
- Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
- Improved seal integrity due to thermal stability of frame
- Alphanumeric grid reference to aid well and sample identification
Use
- Suitable for microscopy and small volume sample storage
- Recommended for low volume PCR
- Compatible with standard multichannel pipettes
- Ideal for use with robotic systems
Options
- Also available as an Individual Access plate (4ti-0970/RA) with individually detachable and sealable wells
- Available barcoded upon request; learn more about our barcoding options
Specifications
| FrameStar 96 Well Skirted Optical Bottom PCR Plate | |
|---|---|
| Plate length | 127.70 ± 0.25mm |
| Plate width | 85.48 ± 0.25mm |
| Plate height | 16.10 ± 0.25mm |
| Well depth | 12 ± 0.10mm |
| Well diameter | 5.46 ± 0.10mm |
| Total well capacity | 180µl |
| Working volume | <0.1ml (100µl) |
| Distance to center of A1 from top edge | 11.24 ± 0.25mm |
| Distance to center of A1 from left edge | 14.38 ± 0.25mm |
| Pitch (distance between A1 and A2) | 9.00mm |
Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes
The surface area of the optical base is 7.74mm2, with a thickness of 0.5mm. The total volume of a conical well is 0.2ml compared to 0.18ml for flat bottom tubes
The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting
Compatibility
AGILENT/STRATAGENE
- Thermal Cyclers (96 well block): Robocycler Gradient
- qPCR Cyclers (96 well block): AriaMx
ANALYTIK JENA/BIOMETRA
- Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
BIO-RAD
- Thermal Cyclers (96 well block): C1000 Touch | S1000
- qPCR Cyclers (96 well block): CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
BIO-RAD MJ RESEARCH
- Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
- qPCR Cyclers (96 well block): Chromo4™ | Opticon2™
EPPENDORF
- Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
- qPCR Cyclers (96 well block): MasterCycler™ ep realplex
GE HEALTHCARE/AMERSHAM
- Sequencers (96 well block): MegaBACE™ 500 | MegaBACE™ 1000 mark 2
PEQLAB/VWR
- Thermal Cyclers (96 well block): peqSTAR 96X
SENSOQUEST
- Thermal Cyclers (96 well block): Labcycler
TECHNE
- Thermal Cyclers (96 well block): TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
- qPCR Cyclers (96 well block): Quantica
For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table and look for the FrameStar® 96 Well Skirted PCR Plate (4ti-0960).
Ordering Information
Part Numbers
Use these part numbers to request a quote, free samples, or to contact an expert:
| FrameStar 96 Well Skirted Optical Bottom PCR Plate Low profile, flat optical bottom, 0.1ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100µl, total well capacity: 180µl | |
|---|---|
| 4ti-0970 | clear wells, clear frame; 50 plates |
Resources
All Resources
APPLICATION NOTES & TECH NOTES
Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium
BROCHURES & SELL SHEETS
PCR Plate-Instrument Compatibility
CATALOGS
Azenta Life Sciences Sample Consumables and Instruments Catalog
TECHNICAL DRAWINGS
FrameStar® 96 Well Non-Skirted PCR Plate, Low Profile Technical Drawing
Application Notes & Tech Notes<
APPLICATION NOTES & TECH NOTES
Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium
Technical Drawings
TECHNICAL DRAWINGS
FrameStar® 96 Well Non-Skirted PCR Plate, Low Profile Technical Drawing
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