FrameStar^® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity

96 well semi-skirted plates designed for use on Roche LightCycler^® 96 & 480 (with 96 well block). Extra white wells improve sensitivity in fluorescent assays. Low profile wells, recommended for reaction volumes <20µl. Rigid frame eliminates warping and distortion during PCR.

PP wells, PC frame; cut corner H12
<100µl working volume, 200µl total well capacity
Available barcoded on request

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Product Details

Key Features

Quality

Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen

Wells

Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
<0.1ml (100µl) working volume, 0.2ml (200µl) total well capacity
Low profile wells, shorter than “standard” profile wells, to decrease the “dead space” between the heated lid of the thermal cycler and the sample in the well
Eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction

Frame

Rigid polycarbonate frame for added mechanical stability
Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
Improved seal integrity due to thermal stability of frame
Alphanumeric grid reference to aid well and sample identification

Use

Compatible with the Roche LightCycler^® 96 and 480 (with 96 well block)
Recommended for low volume PCR
Compatible with standard multichannel pipettes
Ideal for use with robotic systems

Options

Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
Combi packs available with qPCR Seal (4ti-0560)
Available barcoded upon request; learn more about our barcoding options

Specifications

	FrameStar 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity
Plate length	127.70 ± 0.25mm
Plate width	85.48 ± 0.25mm
Plate height	15.60 ± 0.25mm
Well depth	15.10 ± 0.10mm
Well diameter	5.50 ± 0.10mm
Total well capacity	0.2ml (200µl)
Working volume	<0.1ml (100µl)
Distance to center of A1 from top edge	11.24 ± 0.25mm
Distance to center of A1 from left edge	14.38 ± 0.25mm
Pitch (distance between A1 and A2)	9.00mm

Ct values comparison

Ct values of data run on a Roche LightCycler^® 480, comparing 4titude FrameStar Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates
The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct value

	FrameStar Roche Style, High Sensitivity Plate	Competitor R equivalent plates
Ct value	17.23	20.28
Δ Ct	-3.05	0

Compatibility

ROCHE

qPCR Cyclers (96 well block): LC96 | LC480

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information