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FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style

96 well semi-skirted plates with standard profile wells, designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks, without the need for adapters. Rigid frame eliminates warping and distortion during PCR. Ideal for use with robotic systems.

  • PP wells, PC frame; cut corner A12
  • <200µl working volume, 300µl total well capacity
  • Available barcoded on request
  • Ultra-low DNA binding option available

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*Online availability to US only

Low DNA Binding Products
  • Azenta Life Sciences offer low binding products, recommended to support sensitive techniques such as qPCR and Next Generation Sequencing (NGS), as well as storage of samples.
  • Manufactured using selected low-bind polymers, DNA loss is minimized during incubation and sample transfer, making it the ideal solution for use in applications with low DNA input.
  • Low binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics. No contamination or modification of the samples.
  • Tested under a broad temperature range. Maximum DNA recovery after low temperature storage and high temperature incubations.
  • No detectable DNA loss during incubation steps and sample transfer in NGS sample prep and library construction. Ideally suited for sensitive applications with ultra-low nucleic acid input.
Product Details

Key Features

Quality
  • Free from DNase, RNase, human genomic DNA, and endotoxin/pyrogen
Wells
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • <0.2ml (200µl) working volume, 0.3ml (300µl) total well capacity
Frame
  • Cut corner at A12
  • Rigid polycarbonate frame for added mechanical stability
  • Significantly reduced thermal expansion and sample evaporation for improved consistency in PCR results
  • Improved seal integrity due to thermal stability of frame
  • Alphanumeric grid reference to aid well and sample identification
Use
  • Perfect for ABI® thermal cyclers & sequencers
  • Recommended for low volume PCR
  • Ideal for use with robotic systems
  • Compatible with standard multichannel pipettes
Options
  • Available with the following frame color options for the clear well variety: purple, blue, clear, green, red, and black
  • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Also available as a clear frame with frosted wells for use with ABI®/LifeTechnologies® qPCR instruments
  • Ultra-low DNA binding option available (4ti-LB0770/C) for sensitive applications with ultra-low DNA input and for maximum DNA recovery after low temperature storage and high temperature incubation; learn more about our low binding range
  • Ethylene oxide treated option available (4ti-OX770C/SBC) for forensic use; learn more about our ethylene oxide treated range
  • Available barcoded upon request; learn more about our barcoding options
  • FrameStar 96 Lid (4ti-0289) available
Specifications
FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
Plate length124.26 ± 0.25mm
Plate width83.97 ± 0.25mm
Plate height20.70 ± 0.25mm
Well depth20.20 ± 0.10mm
Well diameter5.46 ± 0.10mm
Total well capacity0.3ml (300µl)
Working volume0.2ml (200µl)
Distance to center of A1 from top edge10.495 ± 0.25mm
Distance to center of A1 from left edge12.63 ± 0.25mm
Pitch (distance between A1 and A2)9.00mm
Compatibility
ABI®/LIFE TECHNOLOGIES/THERMO FISHER SCIENTIFIC
  • Thermal Cyclers (96 well standard block): Verity | Proflex | Simpliamp | GeneAmp® 2700/2720/9600/9700
  • qPCR Cyclers (96 well standard block): 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3/5/6/7/12K | ViiA7™
  • Sequencers (96 well block): 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
AGILENT/STRATAGENE
  • qPCR Cyclers (96 well block): Mx3000P™ | Mx3005P™ | Mx4000™
  • Fragment Analyzer Systems: 5200 | 5300 | 5400
ANALYTIK JENA/BIOMETRA
  • Thermal Cyclers (96 well block): Flexcycler2 | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard/Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
  • qPCR Cyclers (96 well block): qTOWER3/G/touch | Toptical
BIOER TECHNOLOGIES
  • Thermal Cyclers (96 well block): Gene Touch 96
BIO RAD
  • Thermal Cyclers (96 well block): C1000 Touch | S1000 | iCycler™ | MyCycler™ | T100
  • qPCR Cyclers (96 well block): MyiQ™ | iCycler™ IQ/IQ 4/IQ 5
BIO-RAD MJ RESEARCH
  • Thermal Cyclers (96 well block): PCT100™/200™/220™/221™/225™/240™
  • qPCR Cyclers (96 well block): Chromo4™
CORBETT RESEARCH
  • Thermal Cyclers (96 well block): (Qiagen) Palm Cycler
EPPENDORF
  • Thermal Cyclers (96 well block): MasterCycler® ep/ep gradient/Pro/Pro S/nexus/nexus gradient/nexus SX1/nexus GSX1
  • qPCR Cyclers (96 well block): Mastercycler™ ep realplex
PEQLAB/VWR
  • Thermal Cyclers (96 well block): peqSTAR 96X
SENSOQUEST
  • Thermal Cyclers (96 well block): Labcycler
TAKARA
  • Thermal Cyclers (96 well block): Dice touch | Gradient
TECHNE
  • Thermal Cyclers (96 well block): Prime | PrimeG | Prime Elite | Prime Elite Satellite | PCRmax Alpha cycler 1/2/4 | TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene

For recommended plate options depending on manufacturer, block type, and instrument please refer to the Azenta Life Sciences Plate Instrument Compatibility Table.

Ordering Information

Part Numbers

Use these part numbers to request a quote, free samples, or to contact an expert:

FrameStar 96 Well Semi-Skirted PCR Plate, ABI® Style
Standard profile, 0.2ml polypropylene wells, polycarbonate frame, cut corner A12; working volume: <200µl, total well capacity: 300µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks
4ti-0770clear wells, purple frame; 50 plates
4ti-0770/Bclear wells, blue frame; 50 plates
4ti-0770/Cclear wells, clear frame; 50 platesBUY ONLINE*
4ti-0770/C/10Pclear wells, clear frame; 10 plates
4ti-0770/Gclear wells, green frame; 50 plates
4ti-0770/Rclear wells, red frame; 50 plates
4ti-0770/Xclear wells, black frame; 50 plates
4ti-0771white wells, black frame; 50 plates
4ti-0772frosted wells, clear frame; 50 plates
4ti-0772/10Pfrosted wells, clear frame; 10 plates
4ti-OX770C/SBC4ti-0770/C for forensic use, ethylene oxide treated, single barcoded; 20 plates
4ti-LB0770/C4ti-0770/C, with low DNA binding properties; 50 plates
Lids
4ti-0289FrameStar 96 Lid (4ti-0289), for use with the FrameStar 96 Well Semi-Skirted Plates, ABI® Style range; 50 lids

*Online availability to US only

Resources

All Resources

APPLICATION NOTES & TECH NOTES
Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

BROCHURES & SELL SHEETS
PCR Plate-Instrument Compatibility

CATALOGS
Azenta Life Sciences Sample Consumables and Instruments Catalog

PUBLICATIONS & POSTERS
Avoiding DNA Adsorption to Tube Walls in NGS Sample Preparation

TECHNICAL DRAWINGS
FrameStar® 96 Well Non-Skirted PCR Plate, Low Profile Technical Drawing

Application Notes & Tech Notes

APPLICATION NOTES & TECH NOTES
Miniaturization of a qPCR Assay with Reduced Reagent Volumes to Achieve Cost Savings in Gene Expression Analyses in Rumen Epithelium

Brochures & Sell Sheet

BROCHURES & SELL SHEETS
PCR Plate-Instrument Compatibility

Catalogs

CATALOGS
Azenta Life Sciences Sample Consumables and Instruments Catalog

Publications & Posters

PUBLICATIONS & POSTERS
Avoiding DNA Adsorption to Tube Walls in NGS Sample Preparation

Technical Drawings

TECHNICAL DRAWINGS
FrameStar® 96 Well Non-Skirted PCR Plate, Low Profile Technical Drawing

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